Supplementary Materials

Dynamics of cortical dendritic membrane potential and spikes in freely behaving rats

Jason J. Moore, Pascal M. Ravassard, David Ho, Lavanya Acharya, Ashley L. Kees, Cliff Vuong, Mayank R. Mehta

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S12
  • Table S1
  • Captions for Movies S1 and S2
  • References

Images, Video, and Other Media

Movie S1
Sample voltage traces of simultaneously-recorded local field potential and dendritic membrane potential. Voltage traces show dendritic membrane potential (DMP) recorded on a tetrode (bottom, red) simultaneously recorded with the cortical local field potential (LFP) on a nearby tetrode (top, blue). Both tetrodes are in the parietal cortex. The sound accompanying the video is a direct translation of the two waveforms, and is in stereo: the left channel is the sound of the LFP and the right channel is the sound of the DMP. Note the different voltage scales for the two traces, demonstrating the large amplitude and inverted polarity of dendritic action potentials (DAP) compared to extracellular spikes.
Movie S2
3-dimensional semi-transparent projection of immunohistologically stained cortical slice. Slice (same image stack as in Extended Data Figure 3a, bottom) is labeled for GFAP (green, putative reactive astrocytes), Iba1 (cyan, putative microglia), and MAP-2 (red, putative dendrites). An oblique dendrite segment trapped within the glial sheath is highlighted in magenta. The dense aggregation of GFAP and Iba1 around the tetrode represents the glial sheath formed by astrocytes and microglia in response to the implanted tetrode.