Supplementary Materials

Self-organized Notch dynamics generate stereotyped sensory organ patterns in Drosophila

Francis Corson, Lydie Couturier, Hervé Rouault, Khalil Mazouni, François Schweisguth

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Supplementary Text
  • Figs. S1 to S13
  • Tables S1 and S2
  • Captions for Movies S1 to S5
  • References

Images, Video, and Other Media

Movie S1
Live imaging of Ac expression. This movie shows an interval of 1.8 h, starting at 5-6 h APF. The midline is near the center, anterior is up, and nuclei are labeled in red (field of view ≈ 350 × 350 μm). Ac (GFP-Ac, green) is first expressed in stripes 1, 3, and 5, then stripes 2 and 4 intercalate between them (cf. Fig 1C-F). The movie ends as stripes 2 and 4 begin to resolve.
Movie S2
Live imaging of Sc expression. This movie shows an interval of 4 h, starting at 5-6 h APF. The midline is near the center, anterior is up, and nuclei are labeled in red. Sc (GFP-Sc, green) is initially expressed in stripe 5, and more faintly in stripes 1 and 3 on each side (cf. Fig 1D). These first stripes later become refined and stripes 2 and 4 intercalate between them. As the movie ends, stripes 2 and 4 are partially resolved.
Movie S3
Live imaging of Dl expression. This movie shows an interval of 2.8 h, starting at 5-6 h APF. The midline is to the left, anterior is up, and nuclei are labeled in red. As the movie begins, the two heminota are migrating towards the midline (suggesting an earlier stage than movies S1 and S2). Dl (Dl-GFP, green) is initially expressed at the locations of proneural stripes 1 and 5, and expression in stripe 3 emerges as the heminota meet and fuse (cf. fig. S3). Stripe 5 gradually narrows from a broad stripe to a narrow file of cells (stripes 1 and 3 are less visible because of tissue folding and uneven contrast).
Movie S4
Live imaging of Notch activity. This movie shows an interval of 2.3 h starting at 5-6 h APF. As in movies S1 and S2, the midline is near the center, anterior is up, and nuclei are labeled in red. Initially, Notch (GFP-m3, green) is activated in a series of bands along the sides of the future stripes 1 and 5 (cf. Fig 1C). Over time, the range of Notch activity around stripes 1 and 5 recedes; a pair of GFP-m3 bands closes in on stripe 5. At the same time, GFP-m3 is expressed in stripe 3, showing the onset of mutual inhibition within the stripe. As the movie ends, isolated cells with low Notch activity, corresponding to emerging SOPs, are visible within the stripes.
Movie S5
Dynamics of the model. This movie shows the same simulation as Fig. 2E, with the same color code (green: inhibitory signal; red/magenta: cell state). As the initial gradient of inhibition recedes, stripes 2-4 emerge and resolve into SOP rows.
 
Correction (28 February 2019): In the fig. S9 caption, the mention of "(A-D)" has been changed to "(A-B)." In table S2, the mention of "fig. S11B,D" (under "Partial reduction in Dl activity") has been changed to "fig. S12B,D"; also, the mention of "fig. S12F" (last line of the table) has been changed to "fig. S9F."
The original version is accessible here.