Supplementary Materials

Thirst-associated preotic neurons encode an aversive motivational drive

William E. Allen, Laura A. DeNardo, Michael Z. Chen, Cindy D. Liu, Kyle M. Loh, Lief E. Fenno, Charu Ramakrishnan, Karl Deisseroth, Liqun Luo

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S9
  • Table S1
  • References

Images, Video, and Other Media

Movie S1
Optogenetic stimulation of MnPO neurons rapidly evokes drinking in water-sated mice The mouse quickly approaches the water cap and begins drinking when blue laser light (5 ms pulses at 20 Hz) is delivered, and the mouse stops drinking after the laser is turned off. Optogenetically-induced drinking was quantified at different stimulation frequencies (Fig. 3E). Note that the mouse does not exhibit undirected gnawing or licking during stimulation.