Supplementary Materials

Multiplexed gene synthesis in emulsions for exploring protein functional landscapes

Calin Plesa, Angus M. Sidore, Nathan B. Lubock, Di Zhang, Sriram Kosuri

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S25
  • Tables S1 to S7
  • References
Tables S8 to S14
Table S8 – DHFR oligos
The oligo sequences used to assemble all 30 libraries of DHFR homologs, the source organism, and corresponding microbead barcodes.

Table S9 – PPAT oligos

The oligo sequences used to assemble all 3 libraries of PPAT homologs, the source organism, and corresponding microbead barcodes.

Table S10 – DHFR homolog info
The assembled gene sequences for DHFR homologs.

Table S11 – PPAT homolog info

The assembled gene sequences for PPAT homologs.

Table S12 – PPAT homolog fitness

The pooled complementation assay fitness values for each PPAT homolog with perfect assemblies.

Table S13 – BMS

The average fitness, number of data points, and standard deviation for all amino acid and position combinations in the PPAT broad mutational scanning (BMS) dataset.

Table S14 – GoF

The fitness for each gain-of-function mutant. Each different mutation is shown on it's own row.

Images, Video, and Other Media

Movie S1
An overview of the DropSynth gene assembly process.