Supplementary Materials

Dentate gyrus mossy cells control spontaneous convulsive seizures and spatial memory

Anh D. Bui, Theresa M. Nguyen, Charles Limouse, Hannah K. Kim, Gergely G. Szabo, Sylwia Felong, Mattia Maroso, Ivan Soltesz

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S20
  • References
Table S1
Intrinsic physiological properties of MCs from opsin- and GFP-expressing MCs in non-epileptic and epileptic mice. N = 5|5|10|5 cells from non-epileptic with opsinjnonepileptic without opsinjepileptic with opsinjepileptic without opsin mice. Values are expressed as mean ± s.e.m.
Table S2
Properties and effect of light delivery on individual juxtacellularly-recorded dentate gyrus neurons in non-epileptic and epileptic, ChR2-expressing and opsin-negative mice.
Table S3
Statistics of optogenetic MC modulation on electrographic seizure duration after and before light or sham trigger. We examined the mean and median seizure duration after or before the trigger, relative light/sham seizure duration, and compared the durations using a nonparametric Mann-Whitney U test.
Table S4
Raw data for object exploration during OLM and ORM training and testing for non-epileptic vs. epileptic mice as well as for eYFP- vs. eNpHR-expressing mice. t L object, time spent exploring left object (s); t R object, time spent exploring right object (s); t total, total time spent exploring both objects; DI, discrimination index (see Experimental procedures); Tr, training; Te, testing; L, light; D, dorsal hippocampus; V, ventral hippocampus. Under Test, test performed is represented as: "task and phase L light on for phase light delivery location". For example, "OLMTr LTe D" represents data for the OLM task during the training phase, light is on during the testing phase, light is delivered to the dorsal hippocampus.