Supplementary Materials

Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding

Alexander B. Rosenberg, Charles M. Roco, Richard A. Muscat, Anna Kuchina, Paul Sample, Zizhen Yao, Lucas Gray, David J. Peeler, Sumit Mukherjee, Wei Chen, Suzie H. Pun, Drew L. Sellers, Bosiljka Tasic, Georg Seelig

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S21
  • Tables S1 to S3, S8, and S11
  • References
Table S4
Top 50 differentially expressed genes in each cluster from the joint brain and spinal cord clustering. Differential expression is calculated as log2(TPMCLUSTER+1)/ log2(TPM~CLUSTER+1), where TPM~CLUSTER is the average TPM for all the cells not in the cluster of interest. We only include genes expressed in at least 20% of the transcriptomes in a cluster.
Table S5
Average expression for each cluster from the joint brain and spinal cord clustering. All values are listed as TPM+1.
Table S6
Genes used to generate P4 sagittal composite ISH maps. Top ten differentially expressed genes from each cluster that were also available in the Allen ISH database for a postnatal day 4 mouse were used.
Table S7
Genes used to generate P14 sagittal composite ISH maps. Top ten differentially expressed genes from each cluster that were also available in the Allen ISH database for a postnatal day 4 mouse were used.
Table S9
Top 50 differentially expressed genes in each cluster from the spinal cord clustering. Differential expression is calculated as log2(TPMCLUSTER+1)/ log2(TPM~CLUSTER+1), where TPM~CLUSTER is the average TPM for all the cells not in the cluster of interest. We only include genes expressed in at least 20% of the transcriptomes in a cluster.
Table S10
Average expression for each cluster from the spinal cord clustering. All values are listed as TPM+1.
Table S12
List of all oligonucleotide sequences used