Supplementary Materials

Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice

M. Brown, F. P. Assen, A. Leithner, J. Abe, H. Schachner, G. Asfour, Z. Bago-Horvath, J. V. Stein, P. Uhrin, M. Sixt, D. Kerjaschki

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S8
  • Captions for Movies S1 to S6
  • References

Images, Video, and Other Media

Movie S1
4T1 tumor cell association with high endothelial venules and blood vessels 2d after intralymphatic infusion. Animation of a semiautomatically segmented light sheet fluorescence microscopy image-stack of a lymph node 2d after intralymphatic infusion of 70.000 mCherry+ 4T1 tumor cells. [blue - segmentation of autofluorescent capsule, red - segmentation of mCherry+ tumor cells, green - segmentation of peripheral node addressin (PNAd+)-labeled HEVs, blue - segmentation of Tomato-lectin-labeled blood vessels, white - segmentation of mCherry+ tumor cells in direct contact with high endothelial venules, pink - segmentation of mCherry+ cancer cells in direct contact with blood vessels].
Movie S2
4T1 tumor cells associate and enwraplymph node blood vessels 3d after intralymphatic infusion. Animation of a semiautomatically segmented time-lapse multiphoton fluorescence image stack of a lymph node 3 d after intralymphatic infusion of 70.000 GFP+ 4T1 tumor cells. (green - native fluorescence of GFP+ cancer cells, white - native fluorescence of Tomato lectin-labeled blood vessels, pink - segmentation and native fluorescence of GFP+ tumor cells in direct contact with blood vessels).
Movie S3
4T1 tumor cell association with high endothelial venules 0d after intralymphatic infusion. Animation of a semiautomatically segmented light sheet fluorescence microscopy image stack of a lymph node at 0d after intralymphatic infusion of 70.000 mCherry-labeled 4T1 tumor cells. (blue - segmentation of autofluorescent capsule, red - segmentation of mCherry+ tumor cells, green - segmentation of peripheral node addressin-labled (PNAd+) high endothelial venules, white - segmentation of mCherry+ tumor cells in direct contact with high endothelial venules).
Movie S4
4T1 tumor cell association with high endothelial venules 1d after intralymphatic infusion. Animation of a semiautomatically segmented light sheet fluorescence microscopy image stack of a lymph node at 1d after intralymphatic infusion of 70.000 mCherry-labeled 4T1 tumor cells. (blue - segmentation of autofluorescent capsule, red - segmentation of mCherry+ tumor cells, green - segmentation of peripheral node addressin-labled (PNAd+) high endothelial venules, white - segmentation of mCherry+ tumor cells in direct contact with high endothelial venules).
Movie S5
4T1 tumor cell association with high endothelial venules 2d after intralymphatic infusion. Animation of a semiautomatically segmented light sheet fluorescence microscopy image stack of a lymph node at 2d after intralymphatic infusion of 70.000 mCherry-labeled 4T1 tumor cells. (blue - segmentation of autofluorescent capsule, red - segmentation of mCherry+ tumor cells, green - segmentation of peripheral node addressin-labled (PNAd+) high endothelial venules, white - segmentation of mCherry+ tumor cells in direct contact with high endothelial venules).
Movie S6
4T1 tumor cell association with high endothelial venules 3d after intralymphatic infusion. Animation of a semiautomatically segmented light sheet fluorescence microscopy image stack of a lymph node at 3d after intralymphatic infusion of 70.000 mCherry-labeled 4T1 tumor cells. (blue - segmentation of autofluorescent capsule, red - segmentation of mCherry+ tumor cells, green - segmentation of peripheral node addressin-labled (PNAd+) high endothelial venules, white - segmentation of mCherry+ tumor cells in direct contact 777 with high endothelial venules).