Supplementary Materials

A method for single-neuron chronic recording from the retina in awake mice

Guosong Hong, Tian-Ming Fu, Mu Qiao, Robert D. Viveros, Xiao Yang, Tao Zhou, Jung Min Lee, Hong-Gyu Park, Joshua R. Sanes, Charles M. Lieber

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S12
  • Tables S1 and S2
  • Captions for Movies S1 and S2
  • References

Images, Video, and Other Media

Movie S1
NIR pupil imaging of mouse eye injected with mesh electronics. This video shows the video-rate NIR imaging and tracking of mouse pupil size changes in response to ambient light intensity modulation. The frame rate is 25 frames per second (fps) and the video is played at 1× real time. The mesh electronics was injected and fixed to the lateral canthus of the eye (right side of the eye), and the pupil boundary is shown as the red dashed circle in the video. This video was taken on Day 1 post-injection of mesh electronics.
Movie S2
Visual stimulation with moving light gratings to a head-fixed mouse. This video shows the setup of moving grating visual stimulation to a mouse restrained in a Tailveiner® restrainer with the head-plate fixed to an optical-table-mounted frame. The frame rate is 30 frames per second (fps) and the video is played at 1× real time. This video covers the duration of gratings moving in eight different directions in one complete stimulation trial. During visual stimulation, single-neuron firing activity of RGCs is recorded through the FFC cable to external recording instrumentation and the size and central location of the pupil are tracked with a NIR camera.