Supplementary Materials

Dual-spindle formation in zygotes keeps parental genomes apart in early mammalian embryos

Judith Reichmann, Bianca Nijmeijer, M. Julius Hossain, Manuel Eguren, Isabell Schneider, Antonio Z. Politi, Maria J. Roberti, Lars Hufnagel, Takashi Hiiragi, Jan Ellenberg

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S10
  • Table S1
  • References

Images, Video, and Other Media

Movie S1
Live-cell time-lapse imaging of MMU x MSP mouse zygote expressing fluorescent TALEs for differential labelling of maternal (magenta) and paternal (cyan) centromeres through distinction of minor and major satellite regions. Chromosome arms are labelled with H2B-mCherry (grey). Time resolution is 7.5 min.
Movie S2
Live-cell time-lapse imaging of MMU mouse zygote expressing EB3-mCherry (green) and tdEos-Cep192 (magenta). Time in min.
Movie S3
Movie S4
Movie S5
Movies S3-5. Live-cell time-lapse imaging of pro-metaphase mouse zygote (phase 2) expressing EGFP-EB3 Left panel: Maximum intensity Z projection. Right panel: The movie has been filtered with a Laplacian of Gaussian (2x2 pixels) to enhance the EB3 tip signal (right panel). Scale bar 10 μm. Time resolution is 800 ms. Movies S3-5 represent the three examples corresponding to Figure S5A.
Movie S6
Movie S7
Movie S8
Movies S6-8.
Live-cell time-lapse imaging of metaphase mouse zygote (phase 3) expressing EGFPEB3 Left panel: Maximum intensity Z projection. Right panel: The movie has been filtered with a Laplacian of Gaussian (2x2 pixels) to enhance the EB3 tip signal (right panel). Scale bar 10 μm. Time resolution is 800 ms.
Movies S6-8 represent the three examples corresponding to Figure S5B.
Movie S9
Movie S10
Movie S11
Movies S9-11.
Live-cell time-lapse imaging of MMU mouse zygote after washout of Nocodazole (> 10 h treatment) expressing αTubulin-EGFP (green) and H2B-mCherry (magenta). Time in min. Movies S9-11 represent three examples corresponding to Figure 3.