Supplementary Materials

Supracellular contraction at the rear of neural crest cell groups drives collective chemotaxis

Adam Shellard, András Szabó, Xavier Trepat, Roberto Mayor

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S20
  • Captions for Movies S1 to S5
  • References

Images, Video, and Other Media

Movie S1
Simulated clusters with uniform contractility: high contractility at both the front and rear (left), or rear contractility only (right). Inner cells are green; outer cells are yellow (contractile) or grey (non-contractile). Movie is 10,000 simulated time-points.
Movie S2
Simulated cluster with no relaxation (continuous rear contraction). Inner cells are green; outer cells are yellow (contractile) or grey (non-contractile). Movie is 5,000 simulated time-points.
Movie S3
Neural crest grafts (magenta) in vivo in wild-type host embryos. Grafts are expressing or not expressing optoGEF-contract (right and left, respectively), and a membrane marker. The green box represents the area of optogenetic activation with the 488-nm laser. Ventral migration is down. Scale bar, 100 μm.
Movie S4
Neural crest grafts (magenta) in vivo in wild-type host embryos. Grafts are expressing or not expressing optoGEF-relax (right and left, respectively), and a membrane marker. The green box represents the area of optogenetic activation with the 488-nm laser. Ventral migration is down. Scale bar, 100 μm.
Movie S5
Neural crest grafts (magenta) in vivo in SDF1-morphant host embryos expressing or not expressing optoGEF-contract (right and left, respectively), and a membrane marker. The green box represents the area of optogenetic activation with the 488-nm laser. Ventral migration is down. Scale bar, 100 μm.