Supplementary Materials

Neonicotinoid exposure disrupts bumblebee nest behavior, social networks, and thermoregulation

James D. Crall, Callin M. Switzer, Robert L. Oppenheimer, Ashlee N. Ford Versypt, Biswadip Dey, Andrea Brown, Mackay Eyster, Claire Guérin, Naomi E. Pierce, Stacey A. Combes, Benjamin L. de Bivort

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S12
  • Tables S1 to S6
  • Captions for Movies S1 to S3
  • References

Images, Video, and Other Media

Movie S1
Time-lapse video of collecting video trials using the robotic platform shown in Fig 1A. Video is shown at 720x speed (two hours of real time footage).
Movie S2
Tracking of BEEtags within a B. impatiens nest after acute imidacloprid exposure. Colors indicate treatment group, with bees in the control, low dose (0.1 ng/bee), and high dose (1.0 ng/bee) groups shown in green, blue, and red, respectively. Bees shown in grey were not removed from the nest for treatment. Frames were recorded at 2 frames per second (fps); playback is at 15fps.
Movie S3
Tracking of foraging behavior of bumblebee (B. impatiens) workers. Time-lapse video 775 shows location and identity (in red for all treatment groups) of foragers visiting a nectar feeder 776 (left) and pollen feeder (right) over the course of 55 minutes.