Supplementary Materials

De novo design of self-assembling helical protein filaments

Hao Shen, Jorge A. Fallas, Eric Lynch, William Sheffler, Bradley Parry, Nicholas Jannetty, Justin Decarreau, Michael Wagenbach, Juan Jesus Vicente, Jiajun Chen, Lei Wang, Quinton Dowling, Gustav Oberdorfer, Lance Stewart, Linda Wordeman, James De Yoreo, Christine Jacobs-Wagner, Justin Kollman, David Baker

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S13
  • Tables S1 and S2
  • Captions for Movies S1 to S5
  • Caption for Data S1
  • References
Data S1
Protein Sequences in Fasta format.

Images, Video, and Other Media

Movie S1
Time-lapse fluorescence microscopy showing DHF58-GFP assembly into filaments in E. coli .
Movie S2
Time-lapse fluorescence microscopy showing the stability of DHF58-GFP filaments upon E. coli lysis by T7 phages.
Movie S3
Attachment of DHF119 filaments to biotinylated anchor proteins DHF119_C6 immobilized on streptavidin-coated slides monitored by TIRF microscopy using YFP fused protomers.
Movie S4
Control Experiment. DHF119 filaments placed over biotinylated anchor proteins for design DHF91 immobilized on streptavidin-coated slides monitored by TIRF microscopy using YFP fused protomers.
Movie S5
Control Experiment. DHF119 filaments placed over streptavidin-coated slides monitored by TIRF microscopy using YFP fused protomers.