Supplementary Materials

Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles

Hae Woong Choi, Jutamas Suwanpradid, Il Hwan Kim, Herman F. Staats, Muzlifah Haniffa, Amanda S. MacLeod, Soman N. Abraham

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S21
  • Captions for Movies S1 to S9
  • References

Images, Video, and Other Media

Movie S1
(Video corresponding to Fig. 2D) 3D visualization of CD301b+ DCs probing the vasculature.
Movie S2
(Video corresponding to Fig. 2E) Intravital two photon microscopy: time-lapse video of antigen uptake by CD301b+ DCs.
Movie S3
(Video corresponding to Fig. S7) Intravital confocal microscopy: time-lapse video of antigen uptake by CD301b+ DCs.
Movie S4
(Video corresponding to Fig. 4A) Intravital confocal microscopy: time-lapse video of antigen-loaded vesicles from perivascular CD301b+ DCs to neighboring MCs and DCs in vivo.
Movie S5
(Video corresponding to Fig. 4B) Microvesicle formation from a DC line (JAWSII)
Movie S6
(Video corresponding to Fig. 4B) Microvesicle migration from a DC line (JAWSII)
Movie S7
(Video corresponding to Fig. S12, 4B) Tracking microvesicle migration from a DC line (JAWSII)
Movie S8
(Video corresponding to Fig. 5A) Microvesicle shedding from a DC line (JAWSII) transfected with NS siRNA
Movie S9
(Video corresponding to Fig. 5A) Inhibition of microvesicle shedding by VPS4A siRNA.