Supplementary Materials

Maternal Huluwa dictates the embryonic body axis through β-catenin in vertebrates

Lu Yan, Jing Chen, Xuechen Zhu, Jiawei Sun, Xiaotong Wu, Weimin Shen, Weiying Zhang, Qinghua Tao, Anming Meng

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Tables S1 and S2
  • Figs. S1 to S18
  • Captions for Movies S1 to S4

Images, Video, and Other Media

Movie S1
Cell movements and morphogenesis of Mhwatsu01sm mutants. Single WT, Class I and Class II Mhwatsu01sm mutant embryos as well as a group of Mhwatsu01sm mutant embryos are observed from the onset of gastrulation throughout 23 hpf. The embryonic shield in WT embryo is indicated by an arrowhead.
Movie S2
Dynamic migration of EGFP-labeled mesodermal cells in Tg(efnb2b:EGFP) and Tg(efnb2b:EGFP);Mhwatsu01sm embryos. Single embryos were observed by confocal microscopy from the gastrulation start to the 18-somite stage. One representative embryo for each group was shown here. Note that GFP-positive mesodermal cells in the Mhwatsu01sm mutant embryo fail to converge dorsally during migration towards the animal pole. The Mhwatsu01sm embryo ends with the onion-like shape. Also see fig. S1.
Movie S3
Dynamic migration of EGFP-labeled endodermal cells in Tg(sox17:EGFP) and Tg(sox17:EGFP); Mhwatsu01sm embryos. Single embryos were observed by confocal microscopy from the 75% epiboly stage to the 18-somite stage. One representative embryo for each group was shown here. The Mhwatsu01sm embryo ends with the calabash-like shape. Note that GFP-positive endodermal cells in the Mhwatsu01sm mutant embryo can migrate towards the animal pole but won’t be able to converge dorsally. Also see fig. S2.
Movie S4
Migration of transplanted GFP-labeled cells in WT and Mhwatsu01sm embryos. (A and B) Transplanted GFP-labeled WT (A) or Mhwa donor cells (B) are able to migrate dorsally to converge in the WT host embryo. (C and D) Transplanted GFP-labeled WT (C) or Mhwa donor cells (D) are unable to migrate dorsally in the Mhwatsu01sm mutant host embryo. Also see fig. S3.