Supplementary Materials

Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

Svetoslav Chakarov, Hwee Ying Lim, Leonard Tan, Sheau Yng Lim, Peter See, Josephine Lum, Xiao-Meng Zhang, Shihui Foo, Satoshi Nakamizo, Kaibo Duan, Wan Ting Kong, Rebecca Gentek, Akhila Balachander, Daniel Carbajo, Camille Bleriot, Benoit Malleret, John Kit Chung Tam, Sonia Baig, Muhammad Shabeer, Sue-Anne Ee Shiow Toh, Andreas Schlitzer, Anis Larbi, Thomas Marichal, Bernard Malissen, Jinmiao Chen, Michael Poidinger, Kenji Kabashima, Marc Bajenoff, Lai Guan Ng, Veronique Angeli, Florent Ginhoux

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Materials and Methods
  • Figs. S1 to S7
  • Captions for Tables S1 to S5
  • Captions for Movies S1 and S2
Table S1
Number of expressed genes in individual cell for Figure S1B.
Table S2
Gene signatures of lung Lyve1loMHCIIhi IMs used in Figure 3A.
Table S3
Gene signatures of lung Lyve1hiMHCIIlo IMs used in Figure 3A.
Table S4
Gene signatures of lung Lyve1loMHCIIhi IMs and mouse orthologs of human transcripts used in Figure 3E.
Table S5
Gene signatures of lung Lyve1hiMHCIIlo IMs and mouse orthologs of human transcripts used in Figure 3E.

Images, Video, and Other Media

Movie S1
Movie S2
WT Cd45.1+ mice were irradiated and reconstituted with bone marrow from either control Lyve1cre/GFP (Movie S1) or Lyve1cre/GFP-Slco2b1flox/DTR (Movie S2) mice. Two months later, they were treated with 1μg/20μL DTx to deplete Lyve1hiMHCIIlo cells, followed by intravenous transfer of 5×106 Ly6Chi inflammatory monocytes purified from ubi-GFP mice. Animals were analyzed 16 hours after transfer. A representative movie of four mice experiment is shown. Three independent experiments were performed.