Supplementary Materials

Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine

Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, Maria del Mar Marquès-Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nollmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S17
  • Captions for Movies S1 to S3
  • References

Images, Video, and Other Media

Movie S1
Example of video showing mEos-ROP6 single molecule imaging used for sptPALM and livePALM analysis.
Movie S2
Video showing GFP-ROP6 localization (10μM NAA, 20 min) in TIRFM before and after bleaching a region of interest containing GFP-ROP6 nanoclusters. Note that GFP-ROP6 nanoclusters are immobile and that they do not recover fluorescence after photobleaching (by contrast to surrounding "non-clustered" GFP-ROP6 signal at the plasma membrane, for which recovery of fluorescence is fast).
Movie S3
Video showing GFP-ROP6 localization (10μM NAA, 20 min) in pss1-3 mutant in TIRFM before and after bleaching a region of interest containing GFP-ROP6 nanoclusters. Note that, like in the WT, GFP-ROP6 nanoclusters are immobile. However, by contrast to the WT, GFP-ROP6 nanoclusters rapidly recover fluorescence after photobleaching.