Supplementary Materials
Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine
Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, Maria del Mar Marquès-Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nollmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais
Materials/Methods, Supplementary Text, Tables, Figures, and/or References
- Materials and Methods
- Figs. S1 to S17
- Captions for Movies S1 to S3
- References
Images, Video, and Other Media
- Example of video showing mEos-ROP6 single molecule imaging used for sptPALM and livePALM analysis.
- Video showing GFP-ROP6 localization (10μM NAA, 20 min) in TIRFM before and after bleaching a region of interest containing GFP-ROP6 nanoclusters. Note that GFP-ROP6 nanoclusters are immobile and that they do not recover fluorescence after photobleaching (by contrast to surrounding "non-clustered" GFP-ROP6 signal at the plasma membrane, for which recovery of fluorescence is fast).
- Video showing GFP-ROP6 localization (10μM NAA, 20 min) in pss1-3 mutant in TIRFM before and after bleaching a region of interest containing GFP-ROP6 nanoclusters. Note that, like in the WT, GFP-ROP6 nanoclusters are immobile. However, by contrast to the WT, GFP-ROP6 nanoclusters rapidly recover fluorescence after photobleaching.
