Supplementary Materials

Multivascular networks and functional intravascular topologies within biocompatible hydrogels

Bagrat Grigoryan, Samantha J. Paulsen, Daniel C. Corbett, Daniel W. Sazer, Chelsea L. Fortin, Alexander J. Zaita, Paul T. Greenfield, Nicholas J. Calafat, John P. Gounley, Anderson H. Ta, Fredrik Johansson, Amanda Randles, Jessica E. Rosenkrantz, Jesse D. Louis-Rosenberg, Peter A. Galie, Kelly R. Stevens, Jordan S. Miller

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S15
  • Table S1
  • Captions for Movies S1 to S5
  • References

Images, Video, and Other Media

Movie S1
Movie compilation to help explain the functional intravascular bicuspid valve design and performance. We first illustrate the design of the bicuspid valve, followed by phase contrast imaging and fluorescence imaging videos of anterograde and retrograde flow of fluorescent beads. The valve is open under anterograde flow and closes under retrograde flow. Note also the mirror image vortices in the sinus region during anterograde flows. This movie supplements Fig. 1.
Movie S2
Movie compilation of fabricated hydrogels with entangled vascular networks. This movie supplements Fig. 2. Perfusion video of red dye injected into helical channel wrapped around axial vessel filled with blue dye. Rotational video of 1°, 2° Hilbert Curve filled with di↵erent colored dyes into individual channels. Animation of layers used for stereolithographic fabrication of torus model, followed by perfusion of red dye through the (3,10) torus knot. Filling of the channels in the torus model with colored dyes enabled μCT acquisition, reconstruction, and visualization of perfusable channels.
Movie S3
Movie compilation illustrating the design and function of the bioinspired alveolar model based on the Weaire-Phelan foam topology. Cyclic ventilation of the airway during RBC perfusion through ensheathing vasculature demonstrates valving of the vasculature by nearby concave airway regions. This movie supplements Fig. 3.
Movie S4
Movie compilation illustrating the design and function of the scalable voronoi lungmimetic model. We show an animation explaining the generative lung topology algorithm where an airway is grown, inlet and outlet vasculature track the airway on opposing sides, and the tips of all branches are then populated with the distal lung subunit. Movie footage of ventilation and perfusion experiments are shown of the distal lung subunit as well as color-filtered views of bidirectional blood flow observed during ventilation and perfusion. This movie supplements Fig. 3.
Movie S5
Luminescence imaging of fabricated tissue constructs perfused with media containing luciferin substrate and either deoxygenated or oxygenated RBCs. Luminescence of entrapped Luc2P-expressing HEK cells is boosted in tissues perfused with oxygenated RBCs. Images from complete time course shown in fig. S10.