Supplementary Materials

Subcellular antibiotic visualization reveals a dynamic drug reservoir in infected macrophages

Daniel J. Greenwood, Mariana Silva Dos Santos, Song Huang, Matthew R. G. Russell, Lucy M. Collinson, James I. MacRae, Andy West, Haibo Jiang, Maximiliano G. Gutierrez

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S4
  • Captions for Movies S1 to S4
  • References

Images, Video, and Other Media

Movie S1
Live-cell imaging of an Mtb-infected hMDM. hMDM infected with Mtb-RFP (green) and treated with 2.5 mg/L BDQ 24 hours post-infection. Following fixation, LD are stained with BODIPY 493/503 (red) and appear yellow due to spectral overlap with RFP. 3D reconstruction from 36 z-planes. Accompanying EM and IM from cell of interest shown in figure 1D.
Movie S2
Live-cell imaging of LD and Mtb burdens. hMDM infected with Mtb-mCherry (red) and stained with BODIPY 493/503 (green). Graph shows total Mtb and LD (normalised to average of uninfected cells) areas in arbitrary units scaled to a starting point of 1. Corresponds to Fig S5 A.
Movie S3
Live-cell imaging of LD and Mtb burdens. hMDM infected with Mtb-mCherry (red) and stained with BODIPY 493/503 (green). Graph shows total Mtb and LD (normalised to average of uninfected cells) areas in arbitrary units scaled to a starting point of 1. Corresponds to Fig S5 B.
Movie S4
Live-cell imaging of LD dynamics. hMDM infected with Mtb-mCherry (red) and stained with BODIPY 493/503 (green). Maximum projection of 8 z-planes. Accompanying stills and quantification in figure 2F. Scale bar is 3 μm.