Supplementary Materials

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes

Chun So, K. Bianka Seres, Anna M. Steyer, Eike Mönnich, Dean Clift, Anastasija Pejkovska, Wiebke Möbius, Melina Schuh

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S26
  • Table S1
  • Captions for Movies S1 to S10
  • References

Images, Video, and Other Media

Movie S1
Confocal sections of a mouse metaphase I spindle stained for the LISD (green, TACC3), aMTOCs (magenta, pericentrin) and microtubules (gray, α-tubulin).
Movie S2
Time-lapse movie of an acutely nocodazole-treated mouse metaphase I oocyte expressing TACC3-mClover3 (green, to label the LISD) and EB3-3×mCherry (magenta, to label microtubules).
Movie S3
Time-lapse movie of fusing spherical condensates in an acutely nocodazole-treated mouse metaphase I oocyte expressing TACC3-mClover3 (gray, to label the spherical condensates).
Movie S4
Time-lapse movie of meiotic maturation of a control mouse oocyte microinjected with mClover3-MAP4-MTBD (green, to label microtubules), CEP192-mScarlet (magenta, to label aMTOCs), H2B-miRFP (blue, to label chromosomes), Trim21 and control IgG.
Movie S5
Time-lapse movie of meiotic maturation of a TACC3-depleted mouse oocyte microinjected with mClover3-MAP4-MTBD (green, to label microtubules), CEP192-mScarlet (magenta, to label aMTOCs), H2B-miRFP (blue, to label chromosomes), Trim21 and anti-TACC3.
Movie S6
Time-lapse movie of meiotic maturation of a TACC3(ΔNT)-TACC3-depleted mouse oocytes microinjected with mClover3-MAP4-MTBD (green, to label microtubules), CEP192- mScarlet (magenta, to label aMTOCs), H2B-miRFP (blue, to label chromosomes), Trim21, anti- TACC3 and TACC3(ΔNT).
Movie S7
Confocal sections of a calcium-treated metaphase I spindle in a control mouse oocyte microinjected with Trim21 and control IgG stained for microtubules (gray, α-tubulin) and chromosomes (magenta, Hoechst).
Movie S8
Confocal sections of a calcium-treated metaphase I spindle in a TACC3-depleted mouse oocyte microinjected with Trim21 and anti-TACC3 stained for microtubules (gray, α-tubulin) and chromosomes (magenta, Hoechst).
Movie S9
Confocal sections of a metaphase I spindle in a control mouse oocyte microinjected with PRC1-3×mClover3, Trim21 and control IgG stained for interpolar microtubules (gray and green, GFP) and K-fibers (magenta, TPX2).
Movie S10
Confocal sections of a metaphase I spindle in a TACC3-depleted mouse oocyte microinjected with PRC1-3×mClover3, Trim21 and anti-TACC3 stained for interpolar microtubules (gray and green, GFP) and K-fibers (magenta, TPX2).