Supplementary Materials

Active cell migration is critical for steady-state epithelial turnover in the gut

Denis Krndija, Fatima El Marjou, Boris Guirao, Sophie Richon, Olivier Leroy, Yohanns Bellaiche, Edouard Hannezo, Danijela Matic Vignjevic

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Theory Note
  • Figs. S1 to S8
  • Table S1
  • Captions for Movies S1 to S4
  • References

Images, Video, and Other Media

Movie S1
Epithelial migration along the villus. Gut explants were derived from Villin:CreERT2/mTmG mouse with mosaic expression of mGFP in the gut epithelium following tamoxifen treatment. The 3D time-lapse movie is showing villus epithelium and stroma (maximum Z projection of 50 μm; Imaris, Surpass, MIP); Time (hh:mm); scale bar, 50 μm.
Movie S2
Recoil after circular laser cut in the lower villus region. Gut explants were derived from myosin IIA-GFP mouse. Circular laser ablation of adherens junctions was performed in the lower villus region. The 2D time-lapse movie shows apical side of the gut epithelium. After denoising, background subtraction, bleach correction and Gaussian blur were applied. Time (mm:ss); scale bar, 20 μm.
Movie S3
Recoil after circular laser cut in the upper villus. Gut explants were derived from myosin IIA-GFP mouse. Circular laser ablation of adherens junctions was performed in the upper villus region. The 2D time-lapse movie shows apical side of the gut epithelium. After denoising, background subtraction, bleach correction and Gaussian blur were applied. Time (mm:ss); scale bar, 20 μm
Movie S4
Epithelium dynamics during migration. Left: Raw images upscaled and denoised prior to skeletonization. Right: Tracking of corresponding skeletonized images. The cells of the green and blue patches could be tracked at all times, black cells delaminate, grey cells are going out of the field or lay at the boundary, white cells are cells newly entered in the field. Newly formed junctions are highlighted in magenta, black and green when they were formed after rearrangements, delamination or division (none), respectively. The blue subpatch highlights a region of interest as it moves and deforms in the villus. Over this time range, junction remodeling and cell intercalation is very limited. Time (hh:mm); scale bar, 20 μm.