Supplementary Materials

DNA loop extrusion by human cohesin

Iain F. Davidson, Benedikt Bauer, Daniela Goetz, Wen Tang, Gordana Wutz, Jan-Michael Peters

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S6
  • Caption for Movies S1 to S10
  • References

Images, Video, and Other Media

Movie S1
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of HeLa cohesin and ATP. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S2
Movie showing extension of gdoubly tethered λ-DNA during perpendicular flow-in of HeLa cohesin, NIPBL-MAU2 and ATP. L
Movie S3
Movie of field of view showing extension of doubly tethered λ-DNAs during perpendicular flow-in of HeLa cohesin, NIPBL-MAU2 and ATP. DNA was visualized by Sytox Orange staining. Scale bar, 10 μm.
Movie S4
Movies showing extension of doubly tethered λ-DNAs (red) during perpendicular flow-in of JF646-labelled HeLa human cohesin (cyan), NIPBL-MAU2 and ATP. (S4) Cohesin binds to DNA and loop extrusion begins at 288 s. (S5) Cohesin binds to DNA and loop extrusion begins at 428 s. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S5
Movies showing extension of doubly tethered λ-DNAs (red) during perpendicular flow-in of JF646-labelled HeLa human cohesin (cyan), NIPBL-MAU2 and ATP. (S4) Cohesin binds to DNA and loop extrusion begins at 288 s. (S5) Cohesin binds to DNA and loop extrusion begins at 428 s. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S6
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of HeLa cohesin, NIPBL-MAU2 and ATP. Loop extrusion begins at 216 s. At 330 s, flow was stopped and restarted in the presence of NIPBL-MAU2 and ATP. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S7
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of HeLa cohesin, NIPBL-MAU2 and ATP. Loop extrusion begins at 188 s. At 396 s, flow was stopped and restarted in the presence of NIPBL-MAU2 and CTP. Loop release begins at 452 s. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S8
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of HeLa cohesin, NIPBL-MAU2 and ATP. Loop extrusion begins at 177 s. At 339 s, flow was stopped and restarted in the presence of ATP. Loop release begins at 450 s. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S9
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of single-chain trimer cohesin, STAG1, NIPBL-MAU2 and ATP. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.
Movie S10
Movie showing extension of doubly tethered λ-DNA during perpendicular flow-in of BMOE-treated single-chain trimer cohesin, STAG1, NIPBL-MAU2 and ATP. DNA was visualized by Sytox Orange staining. Scale bar, 2 μm.