Supplementary Materials

Human cohesin compacts DNA by loop extrusion

Yoori Kim, Zhubing Shi, Hongshan Zhang, Ilya J. Finkelstein, Hongtao Yu

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S8
  • Captions for Movie S1 to S9
  • References

Images, Video, and Other Media

Movie S1
A single-tethered DNA curtain that is homogeneously stained with YOYO-1 and stretched by flow at 0.1 ml min-1.
Movie S2
Compaction of single-tethered DNA by cohesin�NIPBLC.
Movie S3
Injection of a high-salt buffer fully reverses cohesin�NIPBLC-mediated DNA compaction.
Movie S4
YOYO-1 stained 'U'-shaped DNA has a homogeneous intensity profile in the absence of cohesin�NIPBLC.
Movie S5
Loop extrusion of YOYO-1 stained DNA under a constant flow by cohesin� NIPBLC.
Movie S6
Loop extrusion of YOYO-1 stained DNA under a constant flow by cohesin� NIPBLC.
Movie S7
Loop extrusion of YOYO-1 stained DNA under a constant flow by cohesin� NIPBLC.
Movie S8
Rapid DNA loop disruption during a high-salt wash.
Movie S9
Alexa647-labeled cohesin�NIPBLC (magenta) localizes at the base of a DNA loop (green).