Supplementary Materials

Dermal sheath contraction powers stem cell niche relocation during hair cycle regression

Nicholas Heitman, Rachel Sennett, Ka-Wai Mok, Nivedita Saxena, Devika Srivastava, Pieter Martino, Laura Grisanti, Zichen Wang, Avi Ma’ayan, Panteleimon Rompolas, Michael Rendl

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S13
  • Table S3
  • Captions for Movies S1 to S3
  • Captions for Tables S1 and S2
  • References
Table S1
Gene list of DS, DP, DF signatures and overlaps. Gene identities from Venn diagram in Fig. 2D.
Table S2
Gene list of "Regulation and Process of Smooth Muscle Contraction" category. List of gene set of smooth muscle contraction and regulation components used in Gene Set Enrichment Analysis in Fig. 2F

Images, Video, and Other Media

Movie S1
Ex vivo hair follicle contraction. Time-lapse recording of follicle width constriction induced by high K+ spike. Note significant reduction in width above the bulb after three minutes of high K+ (arrows). Also see Figs. 3D, F.
Movie S2
Ex vivo hair follicle contraction blocked by ML7. Time-lapse recording of follicle during high K+ spike in the presence of MLCK inhibitor, ML7. ML7 preincubation blocked contraction with no discernible reduction in follicle width. Also see Figs. 3E, F.
Movie S3
Dynamic cell movements of hair follicle populations during regression captured by intravital imaging. Time-lapse recording of hair follicle regression visualized by tdT, H2BGFP and H2BCer in AcantdT;Tbx18H2BGFP;K14-H2BCer reporter mice. The 5-hour live imaging captures shaft (grey) and DP (red) upward movements concurrent with DS centripetal constriction movement at the club-epithelial strand bottleneck (green arrows).