Supplementary Materials

Transcription factor AP2 controls cnidarian germ cell induction

Timothy Q. DuBuc, Christine E. Schnitzler, Eleni Chrysostomou, Emma T. McMahon, Febrimarsa, James M. Gahan, Tara Buggie, Sebastian G. Gornik, Shirley Hanley, Sofia N. Barreira, Paul Gonzalez, Andreas D. Baxevanis, Uri Frank

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S12
  • Caption for Table S1
  • Captions for Movies S1 and S2
  • Captions for Datasets S1 and S2
  • References
Table S1
Differentially expressed genes between heads and bodies of feeding and sexual polyps. Data are organized into different tabs within the file to delineate tissue specific differential expression between tissues. All values are sorted based on fold change, and genes are described by their top blast hit. This dataset is summarized in Fig. 2.
Dataset S1
Differentially expressed genes and GO term enrichment between somatic cells, i-cells, and germ cells. Dataset is the accumulated results from FAC sorted cells from Tfap2, Piwi1 transgenic reporters. This dataset is summarized in fig. S6.
Dataset S2
DNA sequences of all plasmids used within this study. Each construct contains 5′ promoter elements, the coding region of the fluorescent protein of fluorescent protein Tfap2 fusion, and is flanked by the 3′ non-coding region of each gene.

Images, Video, and Other Media

Movie S1
Live image of transgenic Tfap2 reporter animal. Green region is the germinal zone and is GFP positive in Tfap2::GFP animals. This animal is a female sexual polyp, with mature eggs forming near the bottom of the animal. Timeseries taken over the course of twentyfour hours. Series shows that germ cell production appears to be a slow process (low turn-over).
Movie S2
Live image of migrating Tfap2 wild type cells that carry a reporter transgene in the tissue of a non-fluorescent Tfap2 mutant. This movie shows the transgenic donor animal (bottom) that has wild type Tfap2 alleles. Cells from the donor and can be seen migrating into the Tfap2 mutant tissue (top).