Supplementary Materials

Barcoded microbial system for high-resolution object provenance

Jason Qian, Zhi-xiang Lu, Christopher P. Mancuso, Han-Ying Jhuang, Rocío del Carmen Barajas-Ornelas, Sarah A. Boswell, Fernando H. Ramírez-Guadiana, Victoria Jones, Akhila Sonti, Kole Sedlack, Lior Artzi, Giyoung Jung, Mohammad Arammash, Mary E. Pettit, Michael Melfi, Lorena Lyon, Siân V. Owen, Michael Baym, Ahmad S. Khalil, Pamela A. Silver, David Z. Rudner, Michael Springer

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S19
  • Captions for Tables S1 to S6
  • References
Table S1
List of strains used in this study Bacterial and yeast strains used in this study. List includes wild type strains and mutants generated as part of this study. All unmarked B. subtilis mutations are in-frame deletions generated by Cre-mediated recombination and contain a lox72 scar.
Table S2
List of primers used in this study List of primer sequences used in this study.
Table S3
List of crRNA used in this study List of crRNA sequences used for SHERLOCK reactions in this study.
Table S4
List of barcodes used in this study List of non-redundant barcode sequences used in this study, see fig. S1 for more detailed barcode design.
Table S5
List of barcoded DNA megamers used in this study List of barcoded DNA megamer sequences used for SHERLOCK reactions in this study.
Table S6
Description of incubator experiment Description matrix of conditions used in the incubator experiment, including control conditions, perturbations, and sampling techniques.