Supplementary Materials

Architecture and function of human uromodulin filaments in urinary tract infections

Gregor L. Weiss, Jessica J. Stanisich, Maximilian M. Sauer, Chia-Wei Lin, Jonathan Eras, Dawid S. Zyla, Johannes Trück, Olivier Devuyst, Markus Aebi, Martin Pilhofer, Rudi Glockshuber

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S21
  • Tables S1 to S4
  • Captions for Movies S1 to S7
  • References
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Images, Video, and Other Media

Movie S1
Tomogram of Umod filaments followed by an isosurface of subtomogram average from native Umod. Bars, 100 nm for tomogram, 10 nm for subtomogram average.
Movie S2
Tomogram of eUmod filaments followed by an isosurface of subtomogram average from eUmod. Bars, 100 nm for tomogram, 10 nm for subtomogram average.
Movie S3
Tomogram of Umod filaments incubated with FimHL followed by an isosurface of subtomogram average from Umod-FimHL complex. Bars, 100 nm for tomogram, 10 nm for subtomogram average.
Movie S4
Tomogram of piliated E. coli cell after 4 h incubation with Umod, followed by segmentation of tomogram with E. coli cell in brown, pili in orange, and Umod filaments in blue. Bar, 100 nm.
Movie S5
Tomogram of FIB-milled E. coli - Umod clump shows E. coli cell embedded in mesh of Umod fibers. Bar, 100 nm.
Movie S6
Tomogram of E. coli cell found in urine of patient with urinary tract infection. A pilus â€" Umod contact site is highlighted. Bar, 100 nm.
Movie S7
Tomograms of bacterial cells (Klebsiella, Pseudomonas & Streptococcus) found in urine of patients with urinary tract infections. Bar, 100 nm.