Supplementary Materials

A synthetic synaptic organizer protein restores glutamatergic neuronal circuits

Kunimichi Suzuki, Jonathan Elegheert, Inseon Song, Hiroyuki Sasakura, Oleg Senkov, Keiko Matsuda, Wataru Kakegawa, Amber J. Clayton, Veronica T. Chang, Maura Ferrer-Ferrer, Eriko Miura, Rahul Kaushik, Masashi Ikeno, Yuki Morioka, Yuka Takeuchi, Tatsuya Shimada, Shintaro Otsuka, Stoyan Stoyanov, Masahiko Watanabe, Kosei Takeuchi, Alexander Dityatev, A. Radu Aricescu, Michisuke Yuzaki

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Figs. S1 to S22
  • Table S1
  • Captions for Movies S1 to S8

Images, Video, and Other Media

Movie S1
Gait performance before and after the injection of CPTX in Cbln1-null mice with the C57BL/6J genetic background. Representative movie of gait and footprint of Cbln1-null mice (C57BL/6J genetic background) before (top) or 3 days after (bottom) the injection of CPTX. Related to fig. S8E.
Movie S2
Gait performance after the injection of CPTX in Cbln1-null mice with the ICR genetic background. Representative movie of gait and footprint of Cbln1-null mice (ICR genetic background) 3 days after the injection of Mock (vehicle; top), Cbln1 (middle) or CPTX (bottom). Related to fig. S9A.
Movie S3
Gait performance before and after the injection of CPTX in GluD2-null mice with the C57BL/6J genetic background. Representative movie of gait and footprint of GluD2-null mice (C57BL/6J genetic background) before (top) or 3 days after (bottom) the injection of CPTX. Related to Fig. 3E.
Movie S4
3D view of the restoration of spine density by CPTX in 5xFAD mice. Representative Z stacks and 3D projections with different angles are shown that correspond to Z-projection images from Fig. 4A. Scale bar, 5 μm.
Movie S5
Effects of acute CPTX injections on motor performance at 4 weeks after SCI. Representative movie of free walking mice at 4 weeks after SCI. The mouse that received the CPTX injection immediately after SCI has a red mark on the tail. Related to Fig. 6E.
Movie S6
Effects of acute CPTX injections on the footfall test at 6 weeks after SCI. Representative movie of the footfall test at 6 weeks after SCI. CPTX was injected immediately after SCI. Related to fig. S21A.
Movie S7
Effects of the mock injection at 1 week on the footfall test at 2 weeks after SCI. Representative movie showing the effect of mock injections on the footfall test. Related to fig. S21B.
Movie S8
Effects of the CPTX injection at 1 week on the footfall test at 2 weeks after SCI. Representative movie showing the effect of CPTX injections on the footfall test. Related to fig. S21B.