Supplementary Materials

Engineering synthetic morphogen systems that can program multicellular patterning

Satoshi Toda, Wesley L. McKeithan, Teemu J. Hakkinen, Pilar Lopez, Ophir D. Klein, Wendell A. Lim

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S11
  • Table S1
  • Captions for Movies S1 and S2
  • References
MDAR Reproducibility Checklist

Images, Video, and Other Media

Movie S1
Altering shape of gradient response linked to Fig.1, Fig.3 and Fig.4. Time-lapse movie showing both dynamics and altering interpretation of gradient signaling by engineered cell-cell circuits in Fig.1D, Fig.S2, Fig.3B, Fig.4B and Fig.S8A-B. This movie was taken by IncuCyte with 4x objective at 2hrs/frame for 96hr and 120hr, respectively. In response to gradient signal of synthetic morphogen GFP from the pole region, positive feedback circuits induced signal propagation and formed single domain of active cells in the body region (left). On the other hand, putting opposing inhibitor-secreting pole, two activated and inactivated domains were formed with non-linear transition (right).
Movie S2
Formation of three distinct domains linked to Fig.4. Time-lapse movie showing the dynamics of three domain formation in Fig.4D and Fig.S8D. This movie was taken by Opera Phenix with 10x objective at 2hrs/frame for 72hr. mCherry-PNE morphogen formed narrow BFP+GFP+ domain first, and then GFP morphogen activated positive feedback circuit to induce limited propagation of GFP+ domain, leading to the formation of BFP-GFP+ and BFP-GFP- domain.