Supplementary Materials

Apical stress fibers enable a scaling between cell mechanical response and area in epithelial tissue

Jesús M. López-Gay, Hayden Nunley, Meryl Spencer, Florencia di Pietro, Boris Guirao, Floris Bosveld, Olga Markova, Isabelle Gaugue, Stéphane Pelletier, David K. Lubensky, Yohanns Bellaïche

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S10
  • Tables S1 and S2
  • Caption for Movies S1 to S11
  • References
MDAR Reproducibility Checklist

Images, Video, and Other Media

Movie S1
Laser ablation to estimate tissue stress along the m-l and a-p axis during tissue development. Time-lapse movie of E-Cad:3xGFP tissue upon laser ablation to estimate mechanical stress along the m-l axis (bottom) and the a-p axis (top) at 18 hAPF (left) and 26 h APF (right). Ablation was performed at t=0. Dashed white rectangle outlines the ablated regions. Scale bar: 10μm.
Movie S2
Formation of aSFs in the central domain of the tissue. A-C: Time-lapse movie of E-Cad:3xmKate2 (A,B) and MyoII:3xGFP (A,C) from 18 hAPF to 26 hAPF in the central posterior region of the tissue, where aSFs form (A). Note that aSFs can sometimes be observed to transiently align between adjacent cells and to form multicellular actomyosin structures along the tissue's main stress axis. White box in A indicates the region, which is shown in the close-ups in B and C. Yellow arrowheads indicate aSFs (C). Scale bars: 20μm (A); 10μm (B,C).
Movie S3
aSF laser ablation and cell shape changes. Time-lapse movie of E-Cad:x3mKate2 and MyoII:3xGFP localization upon aSF laser ablation. Dashed white box: ablated region. Time was set to 0s at the time of ablation. Yellow arrowheads: position of the aSF tips prior and upon ablation. Scale bar: 2μm.
Movie S4
AJ laser ablation and cell shape changes. Time-lapse movie of E-Cad:x3mKate2 and MyoII:3xGFP localization upon AJ laser ablation. Dashed white box: ablated region. Time was set to 0s at the time of ablation. Yellow arrowheads: positions of the TCJs prior to and after ablation. Scale bar: 2μm.
Movie S5
AJ ablation followed by aSF ablation. Time-lapse movie of E-Cad:x3mKate2 and MyoII:3xGFP localization upon laser ablation of first the AJ and then the aSF of one cell (around 23s later). Note that no significant aSF reorientation is observed and that the AJ does not heal prior to aSF ablation. AJ time (orange) was set to 0s at the time of AJ ablation, and aSF time (blue) was set to 0s at the time of aSF ablation. Orange and blue dashed box: AJ and aSF ablated regions, respectively. Scale bar: 2μm.
Movie S6
aSF ablation followed by AJ ablation. Time-lapse movie of E-Cad:x3mKate2 and MyoII:3xGFP localization upon laser ablation first of the aSF and then of the AJ of one cell (around 30s later). Note that no significant AJ reorientation is observed and that no aSF healing process was observed in any of the aSF ablations performed. aSF time (blue) was set to 0s at the time of aSF ablation, and AJ time (orange) was set to 0s at the time of AJ ablation. Orange and blue dashed box: AJ and aSF ablated regions, respectively. Scale bar: 2μm.
Movie S7
Multiple aSF ablations in a single cell. Time-lapse movie of E-Cad:x3mKate2 and MyoII:3xGFP localization upon laser ablation of four aSFs within an individual cell. Dashed white box: ablated regions. Time was set to 0s at the time of ablation. Scale bar: 2μm.
Movie S8
Optogenetic clustering of Jub and Wts. (A,B) Time-lapse movie of Wts:GFP (A,B), Cry2Olig:mChFP (A) or Cry2Olig:mChFP:Jub (B) dynamics upon 492 nm light-illumination. Scale bar: 2μm.
Movie S9
Jub and Wts junctional dynamics during aSF movement. (A-B') Time-lapse movie of MyoII:3xGFP (A) and Jub:mKate2 (green, A and fire, A') as well as MyoII:3xmKate2 (B) and Wts:CitFP (green, B and fire, B') localizations during aSF displacement. Yellow arrowheads: Jub:mKate2 or Wts:CitFP clusters. Scale bar: 2μm.
Movie S10
Jub and Wts junctional dynamics during aSF ablation. (A-B') Time-lapse movie of MyoII:mKate2 (A) and Jub:GFP (green, A and fire, A') as well as MyoII:3xmKate2 (B) and Wts:CitFP (green, B and fire, B') localizations upon aSF ablation. Dashed white box: ablated region. Yellow arrowheads: Jub:GFP or Wts:CitFP clusters. Scale bar: 2μm.
Movie S11
aSF nucleation, peeling and breaking. Time-lapse movie of MyoII:3xGFP and E-Cad:3xmKate2 during aSF nucleation at a TCJ (t=0), peeling and breaking at another TCJ (t=42 min). Time was set to 0 min at the time of aSF nucleation (red arrowhead). Yellow arrowheads indicate aSF tips. Note that the aSF breaks at the TCJ. Scale bar: 2μm.