Supplementary Materials
HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells
Haiyang Yu, Shan Lu, Kelsey Gasior, Digvijay Singh, Sonia Vazquez-Sanchez, Olga Tapia, Divek Toprani, Melinda S. Beccari, John R. Yates III, Sandrine Da Cruz, Jay M. Newby, Miguel Lafarga, Amy S. Gladfelter, Elizabeth Villa, Don W. Cleveland
Materials/Methods, Supplementary Text, Tables, Figures, and/or References
- Download Supplement
- Figs. S1 to S15
- Captions for Tables S1 and S2
- Captions for Movies S1 to S4
- References
- Table S1
- A list of key reagents used in this research. Plasmids without an Addgene access number will be deposited with Addgene.
- Table S2
- Raw and processed data of LC-MS/MS results related to Fig 4J.
- MDAR Reproducibility Checklist
Images, Video, and Other Media
- Movie S1
- Live fluorescence imaging of a U2OS cell nucleus expressing TDP-43 anisosomes.
- Movie S2
- Live DIC imaging of a U2OS cell nucleus that shows rapid fusion of both the center and the shell of TDP-43 anisosomes.
- Movie S3
- Live polarization microscopy of a U2OS cell expressing TDP-43 anisosomes. The intensity of each pixel is pseudo-colored to illustrate better contrast.
- Movie S4
- Live DIC imaging of a U2OS cell nucleus that underwent temperature shift (Fig S2)
