Supplementary Materials

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

Haiyang Yu, Shan Lu, Kelsey Gasior, Digvijay Singh, Sonia Vazquez-Sanchez, Olga Tapia, Divek Toprani, Melinda S. Beccari, John R. Yates III, Sandrine Da Cruz, Jay M. Newby, Miguel Lafarga, Amy S. Gladfelter, Elizabeth Villa, Don W. Cleveland

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S15
  • Captions for Tables S1 and S2
  • Captions for Movies S1 to S4
  • References
Table S1
A list of key reagents used in this research. Plasmids without an Addgene access number will be deposited with Addgene.
Table S2
Raw and processed data of LC-MS/MS results related to Fig 4J.
MDAR Reproducibility Checklist

Images, Video, and Other Media

Movie S1
Live fluorescence imaging of a U2OS cell nucleus expressing TDP-43 anisosomes.
Movie S2
Live DIC imaging of a U2OS cell nucleus that shows rapid fusion of both the center and the shell of TDP-43 anisosomes.
Movie S3
Live polarization microscopy of a U2OS cell expressing TDP-43 anisosomes. The intensity of each pixel is pseudo-colored to illustrate better contrast.
Movie S4
Live DIC imaging of a U2OS cell nucleus that underwent temperature shift (Fig S2)