Supplementary Materials

Characterization of a common progenitor pool of the epicardium and myocardium

Richard C. V. Tyser, Ximena Ibarra-Soria, Katie McDole, Satish A. Jayaram, Jonathan Godwin, Teun A. H. van den Brand, Antonio M. A. Miranda, Antonio Scialdone, Philipp J. Keller, John C. Marioni, Shankar Srinivas

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S20
  • Tables S1 to S5
  • Captions for Movies S1 to S14
  • Captions for Data S1 to S4
  • References
MDAR Reproducibility Checklist
Data S1
Table of raw counts for all cells in the unbiased scRNA-seq dataset. Associated metadata is provided in Table S5.
Data S2
Table of raw counts for all cells in the reference scRNA-seq dataset. Associated metadata is provided in Table S7.
Data S3
Metadata corresponding to all the cells sequenced, including individual sample identifiers in the ENA database for the unbiased scRNA-seq dataset.
Data S4
Metadata corresponding to all the cells sequenced, including individual sample identifiers in the ENA database for the reference scRNA-seq dataset.

Images, Video, and Other Media

Movie S1
3D volume rendering movie of a stage 1 cardiac crescent using whole mount immunofluorescence to label the cardiac precursor marker NKX2-5 (red). DAPI labelling of nuclei shown in grey. Related to Figure 1B.
Movie S2
3D volume rendering movie of a stage 1 cardiac crescent using whole mount immunofluorescence to label the contractile protein Sarcomeric Alpha-Actinin (green) and the transcription factor SOX17 (magenta). Sarcomeric Alpha-Actinin marks cardiomyocytes and highlights the cardiac crescent. SOX17 marks the endoderm overlying the heart, and endothelial cells. DAPI labeling of nuclei is shown in grey. Related to Figure 1D.
Movie S3
3D volume rendering movie of a stage 2 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Tbx1 (green), Asb2 (red) and Nkx2-5 (magenta). Related to Figure 2G.
Movie S4
3D volume rendering movie of a stage -1 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Tbx1 (green), Asb2 (red) and Nkx2-5 (magenta). Related to Supplementary Figure 6B.
Movie S5
3D volume rendering movie of a LHT using multiplexed in situ Hybridization Chain Reaction (HCR) to label Tbx1 (green), Asb2 (red) and Nkx2-5 (magenta). Related to Supplementary Figure 6D.
Movie S6
3D volume rendering movie of a stage 2 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Mab21l2 (green), Tbx18 (red) and Nkx2-5 (magenta). Related to Figure 3E.
Movie S7
3D volume rendering movie of a stage 0 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Fst (green), Mab21l2 (red) and Nkx2-5 (magenta). Related to Figure 3F.
Movie S8
3D volume rendering movie of a stage 1 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Vsnl1 (green), Mab21l2 (red) and Fsd2 (magenta). Related to Figure 4B.
Movie S9
3D volume rendering movie of a stage 2 cardiac crescent using multiplexed in situ Hybridization Chain Reaction (HCR) to label Vsnl1 (green), Mab21l2 (red) and Fsd2 (magenta). Related to Supplementary Figure 14B.
Movie S10
3D volume rendering movie of a LHT using multiplexed in situ Hybridization Chain Reaction (HCR) to label Vsnl1 (green), Mab21l2 (red) and Fsd2 (magenta). Related to Supplementary Figure 14C.
Movie S11
3D volume rendering movie of a stage -1 cardiac crescent using whole mount immunofluorescence to label Vsnl1 (green), Mab21l2 (red) and Nkx2-5 (magenta). Related to Figure 4D.
Movie S12
Time-lapse video of a Nkx2-5-Cre; R26-nTnG embryo imaged over a 16-hour period with adaptive light-sheet microscopy showing an Me5 cell (circled) migrating towards the heart from a region rostral to the developing cardiac crescent. As cells migrated towards the cardiac crescent, the cardiac progenitor marker Nkx2-5 was upregulated, as observed by an increase in the expression of nuclear GFP. Circles indicate the same cell tracked across the time-series shown in different channels.
Movie S13
Time-lapse video of a Nkx2-5-Cre; R26-nTnG embryo imaged over a 16-hour period with adaptive light-sheet microscopy showing an Me5 cell (circled) migrating towards the heart from a region rostral to the developing cardiac crescent. As cells migrated towards the cardiac crescent, the cardiac progenitor marker Nkx2-5 was upregulated, as observed by an increase in the expression of nuclear GFP. Circles indicate the same cell tracked across the time-series shown in different channels.
Movie S14
3D volume rendering movie of a early headfold (EHF) embryo using whole mount immunofluorescence to label Fst (green), Mab21l2 (red) and Nkx2-5 (magenta). Related to Figure 5E.