Supplementary Materials

Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts

Jeffrey J. Quinn, Matthew G. Jones, Ross A. Okimoto, Shigeki Nanjo, Michelle M. Chan, Nir Yosef , Trever G. Bivona, Jonathan S. Weissman

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S24
  • Captions for Tables S1 to S4
  • References
MDAR Reproducibility Checklist
Table S1
used for CRISPRi/a experiments in Figs. 5E-F, S18A-B , noting the targeted gene and whether the guide was used for CRISPR-inhibition (i) or -activation (a) experiments. Also included are the sgRNA spacer sequences against cell surface markers used for validating the CRISPRi/a perturbation activity, as in Fig. S18C-F .
Table S2
Tables of metastasis-associated genes identified in the four mouse experiments. Included are the statistics for each gene ( Fig. 5A ), the list of overlapping positive and negative genes identified across all four mouse experiments ( Fig. 5C-D ), and the gene-set used to define the “Metastatic Signature� ( Fig. 6A-B ).
Table S3
Table of metastasis-associated gene signatures identified in mouse M5k. The top 100 gene signatures that are positively correlated with metastatic rate, as in Fig. S16 .
Table S4
Tables of genes in the de novo Hotspot gene modules. Genes identified by Hotspot for Clone #7 (CP007) in Modules