Supplementary Materials

Structure of the human Mediator-bound transcription preinitiation complex

R. Abdella, A. Talyzina, S. Chen, C. J. Inouye, R. Tjian, Y. He

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S13
  • Tables S1 and S2
  • Caption for Movie S1
  • References
MDAR Reproducibility Checklist

Images, Video, and Other Media

Movie S1
Structure of the human Mediator-bound transcription pre-initiation complex.
Movie S2
3D variability analysis of Med-PIC. Top three principal components (rows 1-3) shown of the front (left) and top (right) views of Med-PIC. Mediator and the CAK module of TFIIH largely move as a rigid body relative to the PIC.
Movie S3
3D variability analysis of MedΔTail-PIC. Top three principal components (rows 1-3) shown of the front (left) and top (right) views of MedΔTail-PIC. MedHead, MedΔTail rotates relative to the PIC, and that movement can correlate with the movement of cTFIIH relative to the cPIC.
Movie S4
3D variability analysis of Med-CAK. Top three principal components (rows 1-3) shown of the front (left) and top (right) views of Med-CAK. Mediator can flex, either causing lifting of MedHead and MedMiddle or bringing MedMiddle and MedTail closer together.