Supplementary Materials

Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma

Andrea Cugurra, Tornike Mamuladze, Justin Rustenhoven, Taitea Dykstra, Giorgi Beroshvili, Zev J. Greenberg, Wendy Baker, Zach Papadopoulos, Antoine Drieu, Susan Blackburn, Mitsuhiro Kanamori, Simone Brioschi, Jasmin Herz, Laura G. Schuettpelz, Marco Colonna, Igor Smirnov, Jonathan Kipnis

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Figs. S1 to S9
  • Captions for Tables S1 to S5
  • Captions for Movies S1 to S3
MDAR Reproducibility Checklist
Table S1
List of upregulated and downregulated gene ontology biological processes in GFP+ monocytes compared to GFP monocytes from EAE scRNA-seq.
Table S2
List of differentially expressed genes in GFP+ monocytes compared to GFP monocytes from EAE scRNA-seq
Table S3
List of upregulated and downregulated gene ontology biological processes in GFP+ monocytes compared to GFP monocytes from spinal cord injury scRNA-seq.
Table S4
List of differentially expressed genes in GFP+ monocytes compared to GFP monocytes from spinal cord injury scRNA-seq.
Table S5
List of antibodies used for flow cytometry and immunohistochemistry.

Images, Video, and Other Media

Movie S1
Skull-dura whole-mount of transplanted calvaria flap 30 days after transplantation.
Movie S2
Vascularized channels (white arrow) connecting GFP+ bone marrow to underlying dura after transplantation.
Movie S3
Close-up of ossified channel connecting skull and dura (related to Fig S4K) 30 days after transplantation. Inside the ossified channel (white arrow) GFP+ vasculature (green arrow) is seen originating from the skull and extending towards the dura.