Supplementary Materials

Heterogeneity of meningeal B cells reveals a lymphopoietic niche at the CNS borders

Simone Brioschi, Wei-Le Wang, Vincent Peng, Meng Wang, Irina Shchukina, Zev J. Greenberg, Jennifer K. Bando, Natalia Jaeger, Rafael S. Czepielewski, Amanda Swain, Denis A. Mogilenko, Wandy L. Beatty, Peter Bayguinov, James A. J. Fitzpatrick, Laura G. Schuettpelz, Catrina C. Fronick, Igor Smirnov, Jonathan Kipnis, Virginia S. Shapiro, Gregory F. Wu, Susan Gilfillan, Marina Cella, Maxim N. Artyomov, Steven H. Kleinstein, Marco Colonna

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S16
  • Captions for Tables S1 to S4
  • Captions for Movies S1 and S2
MDAR Reproducibility Checklist
Table S1
Transcriptional signature for each B cell cluster identified by scRNA-seq.
Table S2
Surface markers expression for each B cell cluster identified by CyTOF.
Table S3
Transcriptional signature of dura ABCs identified by scRNA-seq.
Table S4
Complete antibody list used for flow cytometry, immunofluorescence, and CyTOF staining.

Images, Video, and Other Media

Movie S1
Two-photon in vivo imaging of meningeal B cells.
Movie S2
X-ray tomography of the intact mouse skull.