Supplementary Materials

Inhibitors of bacterial H2S biogenesis targeting antibiotic resistance and tolerance

Konstantin Shatalin, Ashok Nuthanakanti, Abhishek Kaushik, Dmitry Shishov, Alla Peselis, Ilya Shamovsky, Bibhusita Pani, Mirna Lechpammer, Nikita Vasilyev, Elena Shatalina, Dmitri Rebatchouk, Alexander Mironov, Peter Fedichev, Alexander Serganov, Evgeny Nudler

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S16
  • Tables S1 to S8
  • Captions for Movies S1 to S4
  • References
MDAR Reproducibility Checklist

Images, Video, and Other Media

Movie S1
Crystal structures of SaCSE in different states and bound to AOAA. The movie shows the X-ray crystal structure of SaCSE in tetrameric and monomeric states and compares the apo SaCSE structure (with PLP not bound to the enzyme covalently, green color) with the structure of the holoenzyme (cyan). In addition, the movie shows the AOAA-bound structure (light orange) superposed with the holoenzyme structure.
Movie S2
Putative druggable areas of SaCSE. The movie depicts druggable areas predicted in silico on the surface view of the tetramer, dimer, and monomer of SaCSE.
Movie S3
Structures of SaCSE bound to lead inhibitors. The movie shows crystal structures of SaCSE bound to NL1-NL3 inhibitors. The protein is in semi-transparent surface representation while inhibitors, Lys-PLP conjugate and adjacent residues are in sticks. The final scene shows superposed structures of the leads.
Movie S4
Rapid pyocyanin coloration of bCSE-deficient P. aeruginosa PA14 upon aeration. The overnight cultures of P. aeruginosa were left without aeration for 1 hour. Brief shaking caused rapid blue/green coloration of the bCSE-deficient (Δ) but not wt cells, that is indicative of the conversion of the transparent reduced form of pyocyanin to its oxidized blue form.