RT Journal Article
SR Electronic
T1 Complement and Hemolytic Antibody: Changes in Their Activity Induced by Mercaptoethanol
JF Science
JO Science
FD American Association for the Advancement of Science
SP 742
OP 743
DO 10.1126/science.147.3659.742
VO 147
IS 3659
A1 Frank, Michael M.
A1 Borsos, Tibor
A1 Rapp, Herbert J.
YR 1965
UL http://science.sciencemag.org/content/147/3659/742.abstract
AB Cell-bound complement components are not destroyed by mercaptoethanol. Destruction of cell-bound hemolytic antibody was prevented by cell-bound complement components. In the absence of cells, the complement components C'3 and C'4 were most susceptible to destruction by mercaptoethanol; C'1, C'1a, and C'2 were most resistant. Mercaptoethanol (0.01M) increases C'1 activity, and this increase is not due to activation of C'1 to C'1a. Destruction of some components required both mercaptoethanol and iodoacetamide treatment.