PT - JOURNAL ARTICLE AU - Keegan, L AU - Gill, G AU - Ptashne, M TI - Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein AID - 10.1126/science.3080805 DP - 1986 Feb 14 TA - Science PG - 699--704 VI - 231 IP - 4739 4099 - http://science.sciencemag.org/content/231/4739/699.short 4100 - http://science.sciencemag.org/content/231/4739/699.full SO - Science1986 Feb 14; 231 AB - The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription. When appropriately positioned in front of a gene these derivatives act as repressors. These and related findings support the idea that GAL4 activates transcription by touching other DNA-bound proteins.