PT  - JOURNAL ARTICLE
AU  - Keegan, L
AU  - Gill, G
AU  - Ptashne, M
TI  - Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein
AID  - 10.1126/science.3080805
DP  - 1986 Feb 14
TA  - Science
PG  - 699--704
VI  - 231
IP  - 4739
4099  - http://science.sciencemag.org/content/231/4739/699.short
4100  - http://science.sciencemag.org/content/231/4739/699.full
SO  - Science1986 Feb 14; 231
AB  - The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription. When appropriately positioned in front of a gene these derivatives act as repressors. These and related findings support the idea that GAL4 activates transcription by touching other DNA-bound proteins.