RT Journal Article SR Electronic T1 A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells JF Science JO Science FD American Association for the Advancement of Science SP 467 OP 470 DO 10.1126/science.3726541 VO 233 IS 4762 A1 Humphries, MJ A1 Olden, K A1 Yamada, KM YR 1986 UL http://science.sciencemag.org/content/233/4762/467.abstract AB Adhesive interactions between cells and the extracellular matrix occur at several stages of metastasis. Such interactions might be inhibited by synthetic peptide probes derived from the cell-binding regions of matrix molecules. Gly-Arg-Gly-Asp-Ser (GRGDS) is a pentapeptide sequence that appears to be critical for cell interaction with fibronectin. Coinjection of GRGDS with B16-F10 murine melanoma cells dramatically inhibited the formation of lung colonies in C57BL/6 mice. Two closely related control peptides, in which specific amino acids within the GRGDS sequence were transposed or substituted, displayed little or no activity. Inhibition by GRGDS was dose-dependent, noncytotoxic, and did not result from an impairment of cellular tumorigenicity. GRGDS may function by inhibiting tumor cell retention in the lung since radiolabeled B16-F10 tumor cells injected with the peptide were lost at a substantially greater rate than control cells.