RT Journal Article
SR Electronic
T1 Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1275
OP 1281
DO 10.1126/science.2531466
VO 246
IS 4935
A1 Huse, WD
A1 Sastry, L
A1 Iverson, SA
A1 Kang, AS
A1 Alting-Mees, M
A1 Burton, DR
A1 Benkovic, SJ
A1 Lerner, RA
YR 1989
UL http://science.sciencemag.org/content/246/4935/1275.abstract
AB A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.