PT  - JOURNAL ARTICLE
AU  - Borrow, J
AU  - Goddard, AD
AU  - Sheer, D
AU  - Solomon, E
TI  - Molecular analysis of acute promyelocytic leukemia breakpoint cluster region on chromosome 17
AID  - 10.1126/science.2218500
DP  - 1990 Sep 28
TA  - Science
PG  - 1577--1580
VI  - 249
IP  - 4976
4099  - http://science.sciencemag.org/content/249/4976/1577.short
4100  - http://science.sciencemag.org/content/249/4976/1577.full
SO  - Science1990 Sep 28; 249
AB  - Acute promyelocytic leukemia (APL; FAB M3) is characterized by a predominance of malignant promyelocytes that carry a reciprocal translocation between the long arms of chromosomes 15 and 17, t(15;17) (q22;q11.2-q12). This translocation has become diagnostic for APL, as it is present in almost 100 percent of cases. A Not I linking clone was used to detect this translocation initially on pulsed-field gel electrophoresis and subsequently with conventional Southern (DNA) analysis. The breakpoints in ten APL cases examined were shown to cluster in a 12-kb region of chromosome 17, containing two CpG-rich islands. The region is the first intron of the retinoic acid receptor alpha gene (RARA).