PT - JOURNAL ARTICLE AU - Harper, JF AU - Sussman, MR AU - Schaller, GE AU - Putnam-Evans, C AU - Charbonneau, H AU - Harmon, AC TI - A calcium-dependent protein kinase with a regulatory domain similar to calmodulin AID - 10.1126/science.1852075 DP - 1991 May 17 TA - Science PG - 951--954 VI - 252 IP - 5008 4099 - http://science.sciencemag.org/content/252/5008/951.short 4100 - http://science.sciencemag.org/content/252/5008/951.full SO - Science1991 May 17; 252 AB - Calcium can function as a second messenger through stimulation of calcium-dependent protein kinases. A protein kinase that requires calcium but not calmodulin or phospholipids for activity has been purified from soybean. The kinase itself binds calcium with high affinity. A complementary DNA clone for this kinase has been identified; it encodes a protein with a predicted molecular mass of 57,175 daltons. This protein contains a catalytic domain similar to that of calmodulin-dependent kinases and a calmodulin-like region with four calcium binding domains (EF hands). The predicted structure of this kinase explains its direct regulation via calcium binding and establishes it as a prototype for a new family of calcium-regulated protein kinases.