PT  - JOURNAL ARTICLE
AU  - Harper, JF
AU  - Sussman, MR
AU  - Schaller, GE
AU  - Putnam-Evans, C
AU  - Charbonneau, H
AU  - Harmon, AC
TI  - A calcium-dependent protein kinase with a regulatory domain similar to calmodulin
AID  - 10.1126/science.1852075
DP  - 1991 May 17
TA  - Science
PG  - 951--954
VI  - 252
IP  - 5008
4099  - http://science.sciencemag.org/content/252/5008/951.short
4100  - http://science.sciencemag.org/content/252/5008/951.full
SO  - Science1991 May 17; 252
AB  - Calcium can function as a second messenger through stimulation of calcium-dependent protein kinases. A protein kinase that requires calcium but not calmodulin or phospholipids for activity has been purified from soybean. The kinase itself binds calcium with high affinity. A complementary DNA clone for this kinase has been identified; it encodes a protein with a predicted molecular mass of 57,175 daltons. This protein contains a catalytic domain similar to that of calmodulin-dependent kinases and a calmodulin-like region with four calcium binding domains (EF hands). The predicted structure of this kinase explains its direct regulation via calcium binding and establishes it as a prototype for a new family of calcium-regulated protein kinases.