PT  - JOURNAL ARTICLE
AU  - Bailey, JE
TI  - Toward a science of metabolic engineering
AID  - 10.1126/science.2047876
DP  - 1991 Jun 21
TA  - Science
PG  - 1668--1675
VI  - 252
IP  - 5013
4099  - http://science.sciencemag.org/content/252/5013/1668.short
4100  - http://science.sciencemag.org/content/252/5013/1668.full
SO  - Science1991 Jun 21; 252
AB  - Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.