RT Journal Article
SR Electronic
T1 Toward a science of metabolic engineering
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1668
OP 1675
DO 10.1126/science.2047876
VO 252
IS 5013
A1 Bailey, JE
YR 1991
UL http://science.sciencemag.org/content/252/5013/1668.abstract
AB Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.