PT  - JOURNAL ARTICLE
AU  - Gloor, GB
AU  - Nassif, NA
AU  - Johnson-Schlitz, DM
AU  - Preston, CR
AU  - Engels, WR
TI  - Targeted gene replacement in Drosophila via P element-induced gap repair
AID  - 10.1126/science.1653452
DP  - 1991 Sep 06
TA  - Science
PG  - 1110--1117
VI  - 253
IP  - 5024
4099  - http://science.sciencemag.org/content/253/5024/1110.short
4100  - http://science.sciencemag.org/content/253/5024/1110.full
SO  - Science1991 Sep 06; 253
AB  - Transposable elements of the P family in Drosophila are thought to transpose by a cut-and-paste process that leaves a double-strand gap. The repair of such gaps resulted in the transfer of up to several kilobase pairs of information from a homologous template sequence to the site of P element excision by a process similar to gene conversion. The template was an in vitro-modified sequence that was tested at various genomic positions. Characterization of 123 conversion tracts provided a detailed description of their length and distribution. Most events were continuous conversion tracts that overlapped the P insertion site without concomitant conversion of the template. The average conversion tract was 1379 base pairs, and the distribution of tract lengths fit a simple model of gap enlargement. The conversion events occurred at sufficiently high frequencies to form the basis of an efficient means of directed gene replacement.