RT Journal Article
SR Electronic
T1 Targeted gene replacement in Drosophila via P element-induced gap repair
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1110
OP 1117
DO 10.1126/science.1653452
VO 253
IS 5024
A1 Gloor, GB
A1 Nassif, NA
A1 Johnson-Schlitz, DM
A1 Preston, CR
A1 Engels, WR
YR 1991
UL http://science.sciencemag.org/content/253/5024/1110.abstract
AB Transposable elements of the P family in Drosophila are thought to transpose by a cut-and-paste process that leaves a double-strand gap. The repair of such gaps resulted in the transfer of up to several kilobase pairs of information from a homologous template sequence to the site of P element excision by a process similar to gene conversion. The template was an in vitro-modified sequence that was tested at various genomic positions. Characterization of 123 conversion tracts provided a detailed description of their length and distribution. Most events were continuous conversion tracts that overlapped the P insertion site without concomitant conversion of the template. The average conversion tract was 1379 base pairs, and the distribution of tract lengths fit a simple model of gap enlargement. The conversion events occurred at sufficiently high frequencies to form the basis of an efficient means of directed gene replacement.