RT Journal Article
SR Electronic
T1 Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1707
OP 1710
DO 10.1126/science.1553558
VO 255
IS 5052
A1 Reynolds, BA
A1 Weiss, S
YR 1992
UL http://science.sciencemag.org/content/255/5052/1707.abstract
AB Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic properties of neurons and astrocytes. Newly generated cells with neuronal morphology were immunoreactive for gamma-aminobutyric acid and substance P, two neurotransmitters of the adult striatum in vivo. Thus, cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.