PT  - JOURNAL ARTICLE
AU  - Kallioniemi, A
AU  - Kallioniemi, OP
AU  - Sudar, D
AU  - Rutovitz, D
AU  - Gray, JW
AU  - Waldman, F
AU  - Pinkel, D
TI  - Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
AID  - 10.1126/science.1359641
DP  - 1992 Oct 30
TA  - Science
PG  - 818--821
VI  - 258
IP  - 5083
4099  - http://science.sciencemag.org/content/258/5083/818.short
4100  - http://science.sciencemag.org/content/258/5083/818.full
SO  - Science1992 Oct 30; 258
AB  - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.