PT - JOURNAL ARTICLE AU - Li, X AU - Wu, Y AU - Zhang, DZ AU - Gillikin, JW AU - Boston, RS AU - Franceschi, VR AU - Okita, TW TI - Rice prolamine protein body biogenesis: a BiP-mediated process AID - 10.1126/science.8235623 DP - 1993 Nov 12 TA - Science PG - 1054--1056 VI - 262 IP - 5136 4099 - http://science.sciencemag.org/content/262/5136/1054.short 4100 - http://science.sciencemag.org/content/262/5136/1054.full SO - Science1993 Nov 12; 262 AB - Rice prolamines are sequestered within the endoplasmic reticulum (ER) lumen even though they lack a lumenal retention signal. Immunochemical and biochemical data show that BiP, a protein that binds lumenal polypeptides, is localized on the surface of the aggregated prolamine protein bodies (PBs). BiP also forms complexes with nascent chains of prolamines in polyribosomes and with free prolamines with distinct adenosine triphosphate sensitivities. Thus, BiP retains prolamines in the lumen by facilitating their folding and assembly into PBs.