PT  - JOURNAL ARTICLE
AU  - Wu, TC
AU  - Lichten, M
TI  - Meiosis-induced double-strand break sites determined by yeast chromatin structure
AID  - 10.1126/science.8290959
DP  - 1994 Jan 28
TA  - Science
PG  - 515--518
VI  - 263
IP  - 5146
4099  - http://science.sciencemag.org/content/263/5146/515.short
4100  - http://science.sciencemag.org/content/263/5146/515.full
SO  - Science1994 Jan 28; 263
AB  - Double-strand DNA breaks (DSBs) occur at recombination hotspots during Saccharomyces cerevisiae meiosis and are thought to initiate exchange at these loci. Analysis of DSB sites in three regions of the yeast genome indicated that breaks occur at or near many potential transcription promoters and that DSBs initiate most, if not all, meiotic recombination. DSB sites displayed deoxyribonuclease I hypersensitivity in chromatin from mitotic and meiotic cells, and changes in chromatin structure produced parallel changes in the occurrence of DSBs. Thus, features of chromatin structure that are established before meiosis play a role in determining where meiotic recombination events initiate.